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Intraarticular (IA) Injection in Mice:

Adenoviruses encoding control shRNA (Ad-shC) or shRNA against ACVR2B (Ad-shACVR2B) were pur chased from Vector Biolabs (Malvern, PA, USA) and IA was injected into mouse knee joints as described previously.[64] After the onset of DMM induced OA, Ad-shACVR2B was administrated once per week via IA injec tion. sACVR2B-Fc was purchased from Y Biologics (Daejeon, Korea) and injected into the knee joints of mice at 4 weeks after DMM surgery (10 μg in a total volume of 10 μL). Control mice received Fc-control (10 μgina total volume of 10 μL; Y Biologics).