Affinity Maturation
Affinity Maturation
Antibodies isolated from a naïve antibody library typically show low antigen-binding affinity and are lightweight. The affinity and stability of an antibody can be improved by optimizing the binding affinity of the antibody. Antibodies developed for specific purposes, such as therapeutic use, require high antigen-binding affinity.
With highly skilled manpower and years of know-how, we achieve affinity maturation by mutational hotspot targeting and LC shuffling, which may be applied alone or in combination.
Our antibody library is highly diverse with more than 1x107 of light chain pools, so it is ideal for discovering antibodies that have undergone affinity maturation.
Affinity Maturation Process
01Y-Biologics’ Strengths in
Affinity Maturation
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Exceptional diversity with more than 1x107 of light chain pools
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Derivation of candidate phage antibodies using Korea’s one and only cutting-edge, high-capacity automated system within 3 months of provision of the antigen and heavy chain DNA
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Quality services provided based on exceptional technology, abundant know-how, and competent professional manpower
02Affinity Maturation Process
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4 WEEKS
Design and production of an antibody humanization library
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1 WEEKS
Biopanning
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4 WEEKS
Monophage analysis
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1 WEEKS
Individual clone selection
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2 WEEKS
lgG conversion
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4 WEEKS
Antibody production
Types of Affinity Maturation
01Light Chain Shuffling
After fixing the heavy chain and adding 106 to 107 of light chains,
a new LC shuffling library is created for biopanning and positive phage selection
02CDR Hotspot Library
+ LC Shuffling
In the case of the DNA of the variable region of the antibody, an LC shuffling library is created for the mutational hotspot where mutations can frequently occur during in vivo affinity maturation for the purpose of biopanning and positive phage selection