Hybridoma Sequencing for Antibody Production
Service Overview
- A service that allows customers to obtain information on antibodies needed in the early stages of antibody drug development from hybridoma cells in their collection
- We have a wide variety of primers that can be used to determine the DNA sequence of a specific monoclonal antibody produced in a hybridoma.
- An antibody whose DNA has been sequenced can be produced in the form of a recombinant antibody at any time, and this can facilitate the management of hybridoma cell lines.
- Recombinant antibody production service entails production of recombinant antibodies in animal cells (HEK293 cells or Expi-CHO cells) based on the DNA sequence of the antibody you have in the backbone of a mouse antibody or a human antibody. We are capable of producing recombinant antibodies with high efficiency using one of our proprietary technologies, Ymax®-tEXPRESS.
Hybridoma Sequencing for Antibody Production
01Y-Biologics’ Strengths in
Hybridoma Sequencing for Antibody Production
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Can determine the DNA sequence of an antibody produced in hybridoma cells
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Can produce recombinant antibodies using Ymax®-tEXPRESS
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Production of antibodies characterized by high purity (>95%) and an endotoxin level of less than 1 EU/ug
02Hybridoma Sequencing Process
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1 DAY
mRNA extraction and RT-PCR
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1 WEEKS
Gene cloning and sequencing
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1 WEEKS
Expression vector cloning and plasmid production
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2 WEEKS
Transient expression and purification of the antibodies
| Services | Products |
|---|---|
| Cloning and sequencing of variable light and heavy chains from hybridomas | Light and heavy chain sequences and T-vector |
| Production of recombinant antibodies after cloning and sequencing of variable light and heavy chains from hybridomas (0.5mg or more) | Light and heavy chain sequences, T-vector, and purified chimeric antibodies |