Cell Line Development
The Starting Point for IND Approval
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- Duration 3 months
- Transfection > RCB candidate screening
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- Monoclonality Beacon platform
- GS (Glutamine Synthetase)
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- Productivity CHOZN GS-/- (~7g/L)
- * Varies depending on the characteristics of the substance submitted by the customer
Cell Line Development Process
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STEP 01
- Cell pool screening
Transfection
2,000 mini pool
Top 2 pools
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STEP 02
- Single cell clone
screening Top 10 clones
Fed-batch evaluation
Top 5 clones
- Single cell clone
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STEP 03
- Cell bank for RCB
candidate Top 5 clones
Cell bank (30vials/clone)
Mycoplasma contamination test
- Cell bank for RCB
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Optional
- Cell stability study
Cell line stability evaluation
Cell density, Cell viability,
Measurement of productivity
Shortened Development Period
Basic cell line development
NEW cell line development
screening
clone
screening
RCB
candidate
Y-Biologics’ Competitive Advantage
Competitive Advantage Secured with the Introduction of Beacon Equipment
- Beacon
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Enables screening for high-expression single cells and monoclonality
Accelerated cell line development for swift development
※ Beacon service available
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30 plates
6 weeks
(96 well plate) -
1 chip
1 week
(1,750 chamber)
MonoClonality
Acquisition of Key Data for IND Approval
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Loading
Transfer of monoclonal cells into a nanopen chamber using OptoElectro Positioning (OEP) technology
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Culture
Continuous imaging in the nanopen chamber to record the cell count and growth speed
* Source: Beacon (Berkely Lights Inc)
Productivity
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High-Expression Clone Screening
Assessment of the productivity of cloning of 30 to 40 cells
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Clone Screening
Productivity of ~7 g/L
Use of State-of-the-Art Equipment for Optimal Results
Cost reduction and improvement of productivity and quality based on an automated process
High-expression single cell selection and monoclonality ensured with the use of state-of-the-art HTS equipment
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The most powerful tool for single cell isolation
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Miniature incubator for fed-batch culture
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Productivity assessment in a 96-well plate
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Increased transfection efficiency and cell stability
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High-definition images of the cell growth process
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Precise inoculation in a 96-well plate