Y-Biologics CRO

Production of Recombinant Antibodies

Optimal High-Efficiency Transient Expression System

Development of an expression vector that is expressed and produced well in HEK293 cells (Ymax®-tEXPRESS system)
High-expression antibody protein production with the Expi-CHO system

Recombinant Antibody Production

Production is possible through synthesis using just the antibody sequence
Productivity, purity, and physical properties confirmed by expression testing within 10 days / 14 days for Expi-CHO
Customized production in small (mg) to large (g) scales (up to 42 L using 6 Multitron units)
If less than 1 L of proteins is required, it can be done within 2 weeks
Can change the isotype of the antibody (human, mouse)

* The development schedule may vary depending on the specifics of the project.

Recombinant Antibody Production Process

DNA
Cloning
Transient
Expression
Purification
Quality Control
Delivery

DNA

Antibody DNA (HC/LC)

DNA sequence-word file

Plasmid : 1㎍

Leader Selection : Y-bio, Other
Only needs to obtain the gene sequence

A.A sequence-word file

Gene synthesis-Outsourcing (4~6 weeks)

Codon-optimization selection : HEK, CHO

Leader Selection : Y-bio, Other

Cloning

Using the User’s Expression Vector: AntibodyHC : 500 / 1L, LC : 500 / 1L (Production scale); DNA PreparationMidi-prep, Purity ＞95%, RNA contamination check

	HEK293F		Expi-CHO
Scale(ML)	HC(㎍)	LC(㎍)	HC(㎍)	LC(㎍)
40	20	20	25	25
1000	500	500	625	625

Using Y-Bio’s Expression Vector: N293F Expression vector

Antibody

Transient
Expression

Expi-CHO Production 4WEEKS

5 DAYS Gene Cloning
Y-Biologics Expression
system
14 DAYS Transfection
Expi-CHO transient
expression
2 DAYS Purification
Affinity chromatography
(Ni-NTA, ProteinA)
1 DAY Quality Control
FPLC & HPLC
1 DAY Delivery
Delivery quick service

Scale	Service	Cell lines	Volume	Timeline	Deliverables
Small Scale	Gene cloning Transient protein expression Purification	Expi-CHO	35~500 ml	3 weeks	Expression data, Purified protein
* Large Scale	Gene cloning Transient protein expression Purification	Expi-CHO	1L~42L	8~12 weeks	Expression report, Purified protein

* In case of large-scale (1 L or more) production, you can purchase the culture medium at an affordable price if you do wish to skip the purification step

HEK293F Production 3WEEKS

5 DAYS Gene Cloning
Y-Biologics Expression
system
6 DAYS Transfection
HEK293F transient
expression
2 DAYS Purification
Affinity chromatography
(Ni-NTA, ProteinA)
1 DAY Quality Control
FPLC & HPLC
1 DAY Delivery
Delivery quick service

Scale	Service	Cell lines	Volume	Timeline	Deliverables
Small Scale	Gene cloning Transient protein expression Purification	HEK293F	40~500 ml	2~3 weeks	Expression data, Purified protein
* Large Scale	Gene cloning Transient protein expression Purification	HEK293F	1L~42L	4~8 weeks	Expression report, Purified protein

* In case of large-scale (1 L or more) production, you can purchase the culture medium at an affordable price if you do wish to skip the purification step.